From Genes to Function: Systematic Approaches Used to Study Candida albicans and Candida glabrata Biology and Pathogenesis
Arnaud Firon and Christophe d'Enfert
from: Candida: Comparative and Functional Genomics (Edited by: Christophe d'Enfert and Bernhard Hube). Caister Academic Press, U.K. (2007)
Genome sequencing has revolutionized the approaches that are used to tackle various aspects of the biology of eukaryotic micro-organisms. In particular, it is now possible to establish collections of mutant strains with systematic inactivation or tagging of all the genes that have been identified through in silico analysis of the genome sequence. Subsequent characterization of the mutant strains in individual tests or in pools using signature tagged mutagenesis screens provides a rapid and systematic evaluation of the contribution of all genes to a particular biological process. Functional genomics, pioneered in S. cerevisiae, is now within reach for Candida albicans, Candida glabrata and possibly other Candida species whose genomes have been sequenced. For example, transposon-mutagenesis or targeted mutagenesis have been used to generate collections of knock-out or conditional mutants in C. albicans and C. glabrata and show considerable promise for the identification of virulence factors as well as novel targets for the development of antifungals. In parallel, complementary information on host-fungus interactions are provided by systematically altering gene function in host cells through methodologies such as RNA interference read more ...