Molecular Epidemiology and Population Structure of Clinically Relevant Burkholderia Species
Tom Coenye, Eshwar Mahenthiralingam and John J. LiPuma
from: Burkholderia: From Genomes to Function (Edited by: Tom Coenye and Eshwar Mahenthiralingam). Caister Academic Press, U.K. (2014)
The aim of this chapter is to provide a comparative overview of the various methods used in epidemiological studies of Burkholderia species, including ribotyping, pulsed-field gel electrophoresis of genomic macrorestriction fragments, randomly amplified polymorphic DNA fingerprinting, Rep- and BOX-PCR fingerprinting, multilocus-based approaches and variable-number tandem repeat analysis. Subsequently, the epidemiology of the most-commonly encountered Burkholderia species is discussed. The focus will be on the Burkholderia cepacia complex and infection in people with cystic fibrosis and the epidemiology of Burkholderia pseudomallei and Burkholderia mallei. Recent advances in our understanding of the population genetics of B. cepacia complex and B. pseudomallei, based on results obtained with multilocus-based approaches like multilocus sequence typing and multilocus restriction typing is also discussed. In a final section we describe the use of whole genome sequencing and typing as novel approach and illustrate its potential with several examples read more ...