Prokaryotic Genome Annotation
Gary Van Domselaar, Morag Graham and Paul Stothard
from: Bioinformatics and Data Analysis in Microbiology (Edited by: Özlem Taştan Bishop). Caister Academic Press, U.K. (2014)
Genome annotation is the process of identifying the important features contained within a genome sequence and attaching relevant biological information to those features. Typically one of the first steps to be applied after sequencing a new genome, annotation involves the coordinated application of a variety of software tools and analysis techniques. An understanding of the tools, databases, computational methods, and available pipelines used to generate genome annotations is necessary to assess their accuracy and their appropriateness for downstream applications. In this chapter we focus on the computational methods that have been developed for annotating bacterial and archaeal genomes. We then survey the popular pipelines that incorporate these methods to generate high quality annotated prokaryotic genomes read more ...