Detecting Active Bacteria Involved in Biodegradation in the Environment
Lee J. Kerkhof and Max M. Häggblom
from: Microbial Biodegradation: Genomics and Molecular Biology (Edited by: Eduardo Díaz). Caister Academic Press, U.K. (2008)
Traditional molecular analyses have led to great understanding of the microbial diversity in natural systems. These approaches can tell the presence of a particular group of bacteria, but does not address the activity. In this chapter we describe molecular methods, including microautoradiography, mRNA analysis, growth assays, and incorporation of stable isotopes, that can be used to determine which bacteria are involved in biodegradation of chemical pollutants. This information should lead to a greater understanding of the role of microbial community structure and function with respect to bioremediation read more ...