In Vitro Isolation of Bat Viruses Using Commercial and Bat-derived Cell Lines
M. Geldenhuys, J. Coertse, M. Mortlock and Wanda Markotter
from: Bats and Viruses: Current Research and Future Trends (Edited by: Eugenia Corrales-Aguilar and Martin Schwemmle). Caister Academic Press, U.K. (2020) Pages: 149-180.
Large viral diversities have been detected from bats using nucleic acid detection methods; however, cell culture isolation of this diversity has been less successful. Virus isolation attempts are limited by challenges that primarily include suitability of available cell lines, quality of infectious viral particles and high levels of biocontainment. In this chapter, we review bat-borne virus isolations in cell culture and factors that may assist in isolation. We review the cell lines that have been used for bat virus isolation, including those derived from non-bat hosts, which are often commercially available, as well as bat-derived cell lines. Additionally, reasons are examined for why specific viruses may be more readily isolated or why certain cell lines may be more useful than others. The chapter focuses on four viral families, namely the Corona-, Paramyxo-, Filo- and Rhabdoviridae; but briefly also mentions the Adeno-, Herpes-, Phenui-, Nairo-, Pox- and Reoviridae read more ...