Peptidoglycan Hydrolases from Phages of Gram-positive Bacteria
Sara Arroyo-Moreno, Colin Buttimer and Aidan Coffey
from: Bacterial Viruses: Exploitation for Biocontrol and Therapeutics (Edited by: Aidan Coffey and Colin Buttimer). Caister Academic Press, U.K. (2020) Pages: 501-536.
Bacterial resistance to conventional antibiotics has become an urgent healthcare issue worldwide. In this light, a renewed interest has emerged for therapy with bacteriophages, which are viruses that can specifically infect bacteria. These viruses encode different proteins that degrade specific bonds within the bacterial cell wall, both to inject their genomic material into the bacterial cells and to release the viral progeny: virion-associated hydrolases and endolysins, respectively. Elimination of Gram-positive pathogens with recombinant versions of phage enzymes represents a promising alternative to antibiotics. In some studies, these enzymes have been used successfully to eliminate/control bacterial pathogens in various anatomical locations in mice and other animal models. This review discusses the strategies developed to improve efficacy in terms of lytic activity and therapeutic delivery of these enzymes read more ...