Laboratory Diagnosis of Arboviruses
Amy J. Lambert and Robert S. Lanciotti
from: Arboviruses: Molecular Biology, Evolution and Control (Edited by: Nikos Vasilakis and Duane J. Gubler). Caister Academic Press, U.K. (2016) Pages: 271-280.
Abstract
In recent years, there have been significant advances in the international capability for the diagnosis of arboviral infections. These advances have predominantly occurred in the field of nucleic acid-based diagnosis; driven by a growing diversity of highly transferable and relatively affordable molecular assays. These comparatively newly described molecular assays complement standard serological assays that continue to be applied preferentially in many laboratories for the detection of arboviral antibodies. In addition to nucleic acid-based and serological efforts, virus isolation remains the standard for arboviral diagnosis and is undertaken most often in cell culture. Here, we summarize a variety of classical and newly developed methods for arboviral diagnosis, including both serological and virus detection techniques. The utility of these approaches for application to human clinical and ecological samples in both diagnostic and research settings is discussed read more ...
