Arbovirus Genomics and Metagenomics
Adam Fitch, Matthew B. Rogers, Lijia Cui and Elodie Ghedin
from: Arboviruses: Molecular Biology, Evolution and Control (Edited by: Nikos Vasilakis and Duane J. Gubler). Caister Academic Press, U.K. (2016) Pages: 175-190.
The arboviruses are a diverse group of arthropod transmitted viruses belonging to several viral families, almost all containing RNA genomes. New methodologies in viral genomics have enabled the probing of the different genomic structures observed in this group, including segmented and non-segmented single and double stranded RNA. We review sequence-dependent targeted gene approaches and sequence-independent metagenomic methods for the characterization of arbovirus genomes. The rapid expansion in the number of arboviral sequencing projects, focused on both known viruses for molecular epidemiology studies and unknown viruses for novel strain discovery, has been enabled by the development of new high throughput sequencing technologies. Outbreaks of both known and novel arboviruses mean that full genome characterization will continue to be salient to both research and healthcare organizations, informing vaccine development while fostering viral pathogenicity studies read more ...