Laboratory Diagnosis and Detection of Alphaviruses
from: Alphaviruses: Current Biology (Edited by: Suresh Mahalingam, Lara Herrero and Belinda Herring). Caister Academic Press, U.K. (2016) Pages: 37-48.
Alphaviruses have been the cause of significant morbidity and mortality in both humans and animals. While infection is suspected clinically it cannot be confirmed without the use of laboratory testing. Laboratory diagnostic techniques have changed dramatically in recent years. The advent of nucleic acid testing has increased our understanding of these viruses and provided a means of rapid diagnosis in the viraemic phase of illness. The development of various ELISA techniques has significantly improved the speed and accuracy with which antibody can be detected, providing an inexpensive means of diagnosing recent infection in both humans and animals. However, the older techniques for both antibody and antigen detection still have value and should not be discarded or relegated to history. This chapter discusses the use of traditional and modern techniques suitable for detecting and diagnosing alphavirus infections read more ...