The Development and Use of Alpha Herpesviruses Expressing Fluorescent Proteins
Gregory A. Smith and Bruce W. Banfield
from: Alpha Herpesviruses: Molecular and Cellular Biology (Edited by: R. M. Sandri-Goldin). Caister Academic Press, U.K. (2006)
The development of fluorescent proteins as tools to monitor gene expression and protein trafficking within living cells and intact organisms has had a tremendous impact on virtually every discipline of the biological sciences including virology. In this chapter we review the development and application of recombinant alpha herpesviruses that express a variety of fluorescent proteins. First, we cover some general considerations, concerns and caveats associated with the use of these viruses. Next, we describe the use of viruses that express fluorescent protein fusions to further understand the trafficking and assembly of subvirion components within living cells. Finally, we discuss the use of recombinant alpha herpesviruses expressing fluorescent proteins to monitor virus infections in experimental animal model systems read more ...