The Development and Use of Microarrays for Analyzing HSVGene Expression
Edward K. Wagner
from: Alpha Herpesviruses: Molecular and Cellular Biology (Edited by: R. M. Sandri-Goldin). Caister Academic Press, U.K. (2006)
The design and construction of long (75mer) oligonucleotide-based DNA microarray for quantitative analysis of Herpes simplex virus transcripts expressed in infected cells and tissues is described. The HSV-1 probes used in the current array are able to specifically cross-hybridize with HSV-2 sequences, and thus, can be used to study both HSV-1 and HSV-2 transcripts. Data presentation and the sensitivity of several methods of detection hybrids on such microarrays are presented. The quantitative measure of HSV transcripts expressed in cells infected with mutations in specific regulatory genes is utilized to illustrate the application of the information gained towards the general understanding of the virus-host interaction read more ...