Acinetobacter baylyi Genetics
L. Nicholas Ornston, Susan Schlimpert, Alison Buchan and Donna Parke
from: Acinetobacter Molecular Biology (Edited by: Ulrike Gerischer). Caister Academic Press, U.K. (2008)
Abstract
The ability of some Acinetobacter strains to undergo natural transformation offers extraordinary advantages for genetic analysis. Now recognized as a member of Acinetobacter baylyi, the Acinetobacter strain designated ADP1 exhibits a robust and resilient metabolism that makes it a useful tool for genetic engineering. The sequenced genome of A. baylyi strain ADP1 raises possibilities for analysis of essential genes. Positive selection of loss-of-function, gain-of-function and change-of-function A. baylyi mutants, particularly when coupled to random PCR-mutagenesis and natural transformation, affords a convenient approach for exploration of how structure influences function in a range of important biological molecules read more ...
