Too much Taq will result in an excessive background of unwanted DNA fragments (a smear on a gel) while a huge excess may cause the reaction to fail with no product being detected. A Taq concentration of 1 unit per 25μl reaction ensures a cleaner product and lower background.
- Real-Time PCR: Advanced Technologies and Applications
- Real-Time PCR in Food Science: Current Technology and Applications
- Quantitative Real-time PCR in Applied Microbiology