Taq Concentration
Recommended reading:
Climate Change and Microbial Ecology | Polymerase Chain Reaction | SUMOylation and Ubiquitination
In a PCR experiment approximately 1 unit of the Taq enzyme should be used for a 25μl reaction. Suboptimal concentration of the Taq enzyme can cause incomplete primer elongation or premature termination of the PCR product synthesis during the elongation step of a PCR cycle.
Too much Taq will result in an excessive background of unwanted DNA fragments (a smear on a gel) while a huge excess may cause the reaction to fail with no product being detected. A Taq concentration of 1 unit per 25μl reaction ensures a cleaner product and lower background.
from PCR Troubleshooting: The Essential Guide see also PCR Troubleshooting and Optimization: The Essential Guide
Further reading
- Real-Time PCR: Advanced Technologies and Applications
- Real-Time PCR in Food Science: Current Technology and Applications
- Quantitative Real-time PCR in Applied Microbiology
See also: Current PCR books
