CIMB Abstract
Curr. Issues Mol. Biol. (2007) 9: 71-86.Assembly of a Functional HCV Glycoprotein Heterodimer
Muriel Lavie, Anne Goffard and Jean DubuissonThe two HCV envelope glycoproteins E1 and E2 are released from HCV polyprotein by signal peptidase cleavages. These glycoproteins are type I transmembrane proteins with a highly glycosylated N-terminal ectodomain and a C-terminal hydrophobic anchor. After their synthesis, HCV glycoproteins E1 and E2 associate as a noncovalent heterodimer. The transmembrane domains of HCV envelope glycoproteins play a major role in E1E2 heterodimer assembly and subcellular localization. The envelope glycoprotein complex E1E2 has been proposed to be essential for HCV entry. However, for a long time, HCV entry studies have remained limited because of the lack of a robust cell culture system to amplify this virus. A few years ago, a model mimicking the entry process of HCV lifecycle has been developed by pseudotyping retroviral particles with native HCV envelope glycoproteins. This model allowed the characterization of functional E1E2 envelope glycoproteins. The data obtained can now be confirmed with the help of a newly developed cellculture system that allows efficient amplification of HCV (HCVcc). Here, we present the recent data that have been accumulated on the assembly of the functional HCV glycoprotein heterodimer.
Access full article: free download
Latest Books
- Chlamydia Biology
- Bats and Viruses
- SUMOylation and Ubiquitination
- Avian Virology
- Microbial Exopolysaccharides
- Polymerase Chain Reaction
- Pathogenic Streptococci
- Insect Molecular Virology
- Methylotrophs and Methylotroph Communities
- Prions
- Microbiota
- Microbial Ecology
- Porcine Viruses
- Lactobacillus Genomics and Metabolic Engineering
- Cyanobacteria
- Viruses of Microorganisms
- Protozoan Parasitism
- Genes, Genetics and Transgenics for Virus Resistance in Plants
- Plant-Microbe Interactions in the Rhizosphere
- DNA Tumour Viruses
- Pathogenic Escherichia coli
- Postgraduate Handbook
- Enteroviruses
- Molecular Biology of Kinetoplastid Parasites
- Bacterial Evasion of the Host Immune System
- Illustrated Dictionary of Parasitology in the Post-Genomic Era
- Next-generation Sequencing and Bioinformatics for Plant Science
- Brewing Microbiology
- Metagenomics
- The CRISPR/Cas System